![]() ![]() The Hill slope term describes the uniformity of the population's state transition from awake to anesthetized. A sigmoidal fit yields the population's ED 50 concentrations for hypnosis as well as its hill slope. By determining the fraction of mice that lose (regain) their righting reflex as a function of increasing (decreasing) anesthetic concentrations, population response curves are easily generated by plotting the fraction of the population that remains responsive vs the log of the anesthetic concentration. As rolling a mouse onto its back is sufficient to awaken a sleeping animal but the constant vestibular bombarding inputs of being upside down are not sufficient to antagonize an anesthetic state, investigators regularly use this nonnoxious behavioral paradigm to assess whether an individual animal retains its perceptive awareness of its surroundings or whether the drug delivery has produced a state of hypnosis. The loss of the righting reflex is convenient and reliable means to indicate the accompanying loss of consciousness. With exposure to hypnotic doses of anesthetic drugs, mice that have been tipped onto their backs in a position of vulnerable dorsal recumbency, lose the protective response to flip back onto all four paws. The loss and subsequent return of the righting reflex is an established behavioral means to assess behavioral responsiveness in rodents ( Franks, 2006). Kelz, in Methods in Enzymology, 2018 3.1 Behavioral Testing ![]()
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